The aim of the present study was to assess the dietary intake of polyunsaturated fatty acids (PUFAs) – through food records – and PUFAs status – through e red blood cell membrane fatty acids composition – in a sample of well-defined sarcopenic older adults according to the recent European Working Group on Sarcopenia in Older People (EWGSOP2) criteria. As the first study to do so in this population, we also aimed to determine the correlation between intake and status of PUFAs. Furthermore, the present study intended to explore associations between dietary intake and nutritional status of PUFAs and individual sarcopenia-defining parameters (muscle strength, muscle mass and physical performance) as well as physical activity and quality of life (QoL).